human dermal fibroblast hdf cells  (ATCC)

Journal: Journal of Biomedical Science

Article Title: Modeling CLN3 Batten disease in astrocytes reveals alterations in mitochondria homeostasis, fatty acid metabolism and oxidative stress response

doi: 10.1186/s12929-026-01253-y

Figure Lengend Snippet: Generation and characterization of iPSC-derived astrocytes. A Schematic overview of astrocyte differentiation from patient-derived iPSCs. Key compounds used to drive differentiation toward a mature astrocyte phenotype are indicated: LIF (leukemia inhibitory factor), CHIR99021, SB431542, CoE, FGF2 (fibroblast growth factor 2), EGF (epidermal growth factor), and CNTF (ciliary neurotrophic factor). B qPCR analysis of astrocyte-specific markers (ALDHL1, GLAST, S100b, GFAP, Vimentin) in iPSC-derived cells. Expression of MAP2 (neuron marker) and OLIG2 (oligodendrocyte marker) was assessed to evaluate cell population purity. C Phase-contrast images and immunocytochemical validation of astrocyte marker expression. Vimentin and GFAP (green), S100β (red), and DAPI-stained nuclei (blue) are shown. D Quantification of CLN3 protein levels in control and CLN3 patient-derived iPSC and astrocytes via targeted mass spectrometry

Article Snippet: Healthy control fibroblast lines (two cell lines) were obtained from ATCC (cat. number PCS-201—012) and the Coriell Institute (cat. number AG05836).

Techniques: Derivative Assay, Expressing, Marker, Biomarker Discovery, Staining, Control, Mass Spectrometry

Journal: Lasers in Medical Science

Article Title: Cellular homeostasis and oncology safety of low pulse frequency laser for photobiomodulation in oral and dermal cells

doi: 10.1007/s10103-026-04887-4

Figure Lengend Snippet: Time-course analysis of cell viability/proliferation following photobiomodulation in ( A ) MCF-7 cells, ( B ) human dermal fibroblasts (HDFs), and ( C ) human gingival fibroblasts (HGFs). Viability is expressed as percentage relative to non-irradiated controls. Irradiation was applied either after 24 h of incubation or immediately after seeding. Continuous doses were used. Data are presented as mean ± standard deviation (triplicates from three independent experiments). Absorbance was measured at 570 nm.

Article Snippet: Human dermal fibroblasts (HDFs; ATCC PCS-201-012) and MCF-7 human breast adenocarcinoma cells (ATCC CRL-3435) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Irradiation, Incubation, Standard Deviation

Journal: Lasers in Medical Science

Article Title: Cellular homeostasis and oncology safety of low pulse frequency laser for photobiomodulation in oral and dermal cells

doi: 10.1007/s10103-026-04887-4

Figure Lengend Snippet: Immunohistochemical staining of human gingival fibroblasts (HGFs) after photobiomodulation (PBM). ( A ) Control (baseline staining). ( B ) Ki-67, showing low proliferative activity. ( C ) FAK, with cytoplasmic and focal adhesion-associated staining. ( D ) Integrin β1, with increased cytoplasmic and membranous expression. ( E – F ) COX-1 and COX-2, showing minimal or absent staining. PBM was applied at 808 nm, 100 mW, delivering 2 J/cm² at 1 cm distance. Images were acquired at 40× magnification (scale bar = 15 μm) and are representative of three independent experiments.

Article Snippet: Human dermal fibroblasts (HDFs; ATCC PCS-201-012) and MCF-7 human breast adenocarcinoma cells (ATCC CRL-3435) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Immunohistochemical staining, Staining, Control, Activity Assay, Expressing